"Enough is enough. Once I m back to India I will initiate proceedings of criminal defamation," Fadnavis, on a week-long trip to America to woo investment to the state, tweeted.
However, he did not specify against whom he plans to take legal action.
"I reiterate, the reports of me delaying flight are misleading. In fact when I'd already boarded, how can I say I won't travel without delegation?" said Fadnavis on Twitter.
As per the Air India, flight AI191 to Newark from Mumbai was delayed by 57 minutes partly due to technical/operational reasons, and also the ATC.
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The alleged incident took place on Monday when Fadnavis was leaving on a week-long visit to the US along with Industries Minister Subhash Desai, Chief Secretary Swadheen Kshatriya and Principal Secretary Pravin Pardeshi.
Congress has targeted Fadnavis over the issue, with former Chief Minister Prithviraj Chavan demanding that he apologise if he had caused the delay.
"If Fadnavis did request for delaying the flight, then he must apologise. Nobody can delay the flight except for safety reasons. By interfering with the flight procedure, Fadnavis inconvenienced the hundreds of passengers on board," Chavan, a senior Congress leader said, told PTI.