"They took out a peaceful tractor rally from Vitthalpur in Ahmedabad to Gandhinagar," Ahmedabad (Rural) Superintendent of Police Gagandeep Gambhir told PTI.
Farmers from 44 villages, mainly hail from Mandal, Viramgam and Becharaji talukas of Ahmedabad and Mehsana districts.
Driving more than 500 tractors, they were accompanied by rally organisers in 100 cars and as many motorcycles.
Among those supporting the farmers were former Gujarat finance minister Sanat Mehta, former BJP MLA Kanu Kalsariya, who spearheaded the agitation against Nirma's cement plant at Mahuva in Bhavnagar, former Planning Commission member Y K Alagh, besides social activists.
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"We have stated in our memorandum that the Gujarat state government should cancel its notification to develop Mandal-Becharaji as a Special Investment Region," Convener of the Jameen Adhikar Andolan Gujarat (JAAG) Sagar Rabari said.
The memorandum also stated that farmers are unwilling to part with their fertile land for the proposed project.
The SIR covers a Maruti car plant located at Hansalpur village on Viramgam-Becharaji highway and the Gujarat government plans to develop it as an auto and knowledge hub.