The fortification dates to the reigns of Kings David and Solomon in the 10th century BCE.
The fabled mines of King Solomon were believed to be located among copper smelting camps in Israel's Timna Valley.
The arid conditions at Timna have seen the astonishing preservation of 3,000-year-old organic materials, which have provided archaeologists at Tel Aviv University with a unique window into the culture and practices of a sophisticated ancient society.
"According to the Bible, David travelled hundreds of miles outside of Jerusalem and engaged in military conflict in the desert - striking down '18,000 Edomites in the Valley of Salt'," said Ben-Yosef.
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"Having found evidence of defensive measures - a sophisticated fortification - we understand what must have been at stake for him in this remote region: copper," he said.
"Copper was a rare product and very challenging to produce," Ben-Yosef said.
"The discovery of the fortification indicates a period of serious instability and military threats at that time in the region," he said.
In the remarkably intact two-room fortification, located in one of the largest smelting camps in the Timna Valley, the researchers also found evidence of a complex long-distance trade system that probably included the northern Edomite plateau, the Mediterranean coastal plain and Judea.
The complex featured pens for draught animals and other livestock.
According to precise pollen, seed and fauna analyses, they were fed with hay and grape pomace - high-quality sustenance that must have been delivered from the Mediterranean region hundreds of miles away.
"We found animal bones and dung piles so intact, we could analyse the food the animals were fed with precision. The food suggests special treatment and care, in accordance with the key role of the donkeys in the copper production and in trade in a logistically challenging region," he said.
The research was published in The Journal of Archaeological Science: Reports.
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