The accident occurred yesterday near the Laquecahue airfield in the Bio Bio region at approximately 1700 GMT.
Four passengers -- a woman and three men -- died after the plane had a troubled landing, said Humberto Toro, the governor of Arauco, noting the region's high winds.
It was not clear if the pilot was killed.
The aircraft, which was owned by a private company, was flying the Mocha Island-Tirua route about 720 kilometres south of Santiago, the official told a Chilean television network.
Investigations into the incident's cause are underway.