The pint-sized Jamaican, unmissable with a yellow flowered garland on top of her bright green braids, shot out of the blocks and eased home, right arm raised, in 10.76 seconds.
Dutchwoman Dafne Schippers took silver in a national record of 10.81sec with American Tori Bowie claiming bronze (10.86) in the climax to day four at the world championships in Beijing.
Double Olympic champion Fraser-Pryce also won world gold in 2009 and 2013, and sealed her third title a day after Bolt won a thrilling 100m duel with America's Justin Gatlin.
"When I ran the heats, I remembered when back in 2008 at the Olympic Games I was 21 years old," Fraser-Pryce said.
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"I expected nothing then. I came out here again tonight -- with a gold medal."
Fraser-Pryce, however, was outdone by Ezekiel Kemboi, the flamboyant Kenyan who held his nerve for a remarkable fourth consecutive world gold in the 3000m steeplechase -- an event he has dominated for the best part of 11 years.
"I'm so happy about my fourth consecutive title," said Kemboi, who attacked the final hurdle like a high hurdler when he sensed team-mate Conseslus Kipruto closing on him down the final straight.
"The race was really fast. On the last lap nobody could follow me. I will be celebrating tonight with my team-mates."
Kemboi also won Olympic golds in 2004 and 2012, and was clear what his aim for next year would be.
"My next big goal, of course, are the Rio Olympic Games," the 33-year-old said.
Another champion to defend their title was Colombia's Caterine Ibarguen, who leapt 14.90 metres for triple jump gold and stretched her amazing winning streak to 29 competitions.
There was a second Kenyan track gold when Vivian Cheruiyot regained the women's 10,000m title, producing a commanding last-lap surge for victory just a year after starting a family.