The latest round of talks begins days after the Islamic State jihadist group was forced out of its de facto capital Raqa in northern Syria, in a major victory for the US-backed Kurdish-Arab Syrian Democratic Forces.
Recent rounds of talks in the Central Asian nation have focused on ironing out the details of a Russia-led plan for four de-escalation zones in Syria.
"Closed-format talks have begun," Kazakh foreign ministry spokesman Anuar Zhainakov told AFP, adding that the two-day negotiations would conclude with statements to the press.
Despite backing opposite sides in the war, Ankara and Moscow have been working closely on Syria since a 2016 reconciliation ended a crisis caused by the shooting down of a Russian war plane.
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A de-escalation zones plan was first tabled in Astana in May to minimise fighting between government forces and moderate rebel factions, as well as improve access for aid for civilians living in the zones.
But international organisations painted the humanitarian situation in Eastern Ghouta -- covered by the zones deal and located just outside the capital Damascus -- in dire terms earlier this month.
While the de-escalation zones brought about an initial reduction in fighting, the International Red Cross has voiced concern that the violence has intensified again, including in the zones.
Previous rounds of Astana negotiations have ended without major breakthroughs, but they have made more progress than the parallel UN-driven talks on Syria in Geneva.
The last round of talks in September saw Russia, Turkey and Iran agree to jointly police a buffer zone in the contentious northern province of Idlib, where Ankara and Tehran are viewed as having competing interests.
Syria's United Nations envoy Staffan de Mistura urged parties to move on from the zones towards "a more stable political settlement" during a mid-October visit to Moscow.
The next round of Geneva talks will begin on November 28, De Mistura has said.
Russia's military intervention two years ago in the Syrian war that has claimed more than 330,000 lives to date turned the tables in Assad's favour.
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