Coming into the game with a series of poor scores behind him, the Delhi left-hander showed glimpses of his old self with a delightful knock of unbeaten 60 runs off 107 balls, consisting of eight scintillating boundaries, at the KSCA Stadium.
This is Gambhir's 51st first-class half century of his career.
Gambhir walked into the middle with great determination and focussed himself on not playing any rash strokes which was his undoing in Shimoga Test.
Pujara, who also had a poor run in the series after getting a century against South Africa A, was batting on 36 off 58 balls with four hits to the fence.
Resuming at their overnight score of 10 for one, India had a poor start to the day as they lost opener V A Jagadeesh early on when he was castled by Trinidadian left-arm fast bowler Delorn Johnson for 16.