Justice Mukta Gupta accepted the prosecution argument that the investigation in a preliminary stage and turned down Yadav's plea, saying "no interference is required into the order of trial court."
Challenging the trial court's March 12 order dismissing his bail petition, Yadav, 35, in his plea claimed he was in no way connected to the incident.
Yadav, arrested since January 2, sought bail on the grounds of parity saying co-accused Rakam Singh has already been granted bail in the case.
The 23-year-old girl was gangraped in the moving bus in south Delhi and died in a hospital in Singapore on December 29.