Wessal Abu Alyan, a 40-year-old mother of four, said playing helped her "feel free and strong".
"I try to ignore the negative looks from people toward the disabled, but I know there's still a long way to go before we are completely integrated in society," she said.
As the women practiced, they were given advice and encouragement from Jess Markt, an American who began playing wheelchair basketball after a spinal cord injury when he was 19.
In Gaza at the invitation of the International Committee of the Red Cross (ICRC), he is overseeing 115 players, both men and women, over the course of three weeks.
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"They've made real progress," said Markt, who had also worked with the players last year.
The programme has particular relevance in the Hamas-run Gaza Strip, which has seen three wars with Israel since 2008.
More than 75,000 Gazans out of a population of 1.9 million are physically or visually disabled, according to the ICRC.
Of those, a third were wounded by war.
Suhair Zakkout, ICRC spokeswoman in Gaza, said the players could help "show that Gaza is not only war and violence".
They can "become ambassadors in international competitions."
Money shortages, however, have hampered their efforts, while cultural expectations of women have also been an obstacle in the Islamist-run Palestinian territory.
"There is the social pressure that sometimes says women shouldn't play sports," Markt said.
"I think there is a lot of pressure here, and it's difficult, but I think these women just like those in other countries can overcome that pressure and set an example for other girls to come and start to play.