The firm said it had made "auction revenues of USD 36.5 million," during the sale last week "a record for any Gemfields auction."
Last April, the Zambian government banned auctions abroad of its gems, prompting anger among buyers.
The auctions previously took place in Jaipur in India, London, Johannesburg and Singapore.
"Zambian gemstones have for a long time been sold on foreign markets, a situation that has contributed to capital flight and denied Zambians of the much-needed benefits from the resource," the ministry of mines said at the time.
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The firm had earned USd 96.4 million in four auctions in Zambia, to beat its previous revenue record only eight months into the financial year, said CEO Ian Harebottle.
"We have achieved two new records - the highest aggregate revenue and the highest average per carat price."
Bidders paid USD 59.31 per carat on average.
Buyers from the United States, Germany and elsewhere attended the latest sale of gems from the Kagem Mine north of Lusaka, which is 75-per cent owned by Gemfields.