Gillard walked on stage this morning at Sydney's Customs House to launch a cyber safety initiative when she slipped out of one of her high heels.
Gillard reportedly was heard telling a male guest who helped her out to put the shoe back as sayinh "it is a Cinderella moment".
It is the second time Gillard has shed a shoe in public.
Earlier, she lost one on Australia Day this year as she was hustled out of a Canberra restaurant by her security staff when the restaurant was surrounded by Aboriginal protesters.
The shoe was said to be initially posted for sale on eBay but members of the Aboriginal tent embassy returned her missing blue suede shoe to Parliament House days later.