Deogarh District & Sessions Court (first) Judge, Pankaj Srivastav rejected the petition a week after he issued arrest warrants against Singh.
Earlier, a Bokaro court had also rejected Singh's anticipatory bail on similar charges but Jharkhand High Court quashed it yesterday.
The warrants were issued after an FIR was filed against the BJP leader for his alleged inflammatory speech on April 18 saying those who opposed BJP's prime ministerial candidate Narendra Modi would have to find a place in Pakistan.
Singh had repeated the alleged hate speech at the Patna airport on April 20 and a FIR was issued by Patna administration at the instance of the Election Commission.