The moment captured by cameras left everyone guessing about his peculiar expression and whether the nudge by Gaga was intentional.
A user joked about DiCaprio's gesture, writing on Twitter, "Watch Leonardo DiCaprio react to a woman older than 25."
While another wrote, "Let's just appreciate Leonardo DiCaprio's facial expressions tonight."
"My favourite moment so far at the #Goldenglobes #LeonardoDiCaprio #LadyGaga," designer Zac Posen wrote about it on Instagram.
The 29-year-old pop star won the award for her debut show "American Horror Story", while DiCaprio registered his third Golden Globe win for "The Revenant".