After listing at Rs 717, up 30.36 per cent over the issue price, shares of the company later ended at Rs 824.15, a steep rise of 49.84 per cent on BSE.
During the day, it zoomed 53.16 per cent to Rs 842.40.
On the NSE, the stock witnessed a sharp rally of 50 per cent to Rs 825 at close.
"Newly listed Dr Lal PathLabs remained in limelight today after it listed on the bourses with handsome gains", said Achin Goel, Head: Wealth Management & Financial Planning, Bonanza Portfolio Ltd.
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The company's market valuation stands at Rs 6,811.17 crore.
The stock market today witnessed another listing from drug maker Alkem Laboratories that surged nearly 32 per cent on its debut trade.
Dr Lal's IPO was a big success with 33.41 times over- subscription.
In the IPO, the qualified institutional buyers (QIBs) part was subscribed 63.56 times and non-institutional investors saw 61.28 times subscription.
The retail investors category was subscribed 4.24 times.
Prior to Dr Lal's IPO, Alkem, S H Kelkar, VRL Logistics, Power Mech Projects and Syngene International have seen strong response in terms of subscription so far this year.
This year has seen hectic fund raising activities in the IPO segment despite a flat market. Generally, an IPO boom follows a robust equity market.
In the broader market, the Sensex ended at 25,850.30, up 259.65 points.