Domingos-Antonio Gomes beat the previous record by 59 hits, after spending about four months training for the challenge in Portugal.
His technique involved using two alternate fingers to hit the same key, allowing him to play the note at the speed of about 13 hits per second.
"I am a Portuguese-American professional pianist and have been playing since the age of seven," Gomes told Guinness World Records.
"I broke this record in order to gain visibility in the international world of music by demonstrating my athletic prowess on the piano," he said.