The massacre took place yesterday at the Las Dominicanas M&M Hair Salon, which is situated in a strip mall in Casselberry, north of Orlando.
36-year-old Bradford Baumet burst into the Dominicanas Hair Salon and started shooting, WESH TV reported.
Three women died at the scene, while a fourth was taken to a hospital, authorities said.
Officials with the Seminole Fire Department said employee Noelia Gonzalez-Brito, 28; customer Gladys Cabrera, 52; and Salon co-owner Eugenia Marte, 45, were shot and killed at the salon.
Marcia Santiago, 44, was taken to Orlando Regional Medical Center. She was said to be in a serious condition, and two other women escaped the shooting by hiding in the bathroom.
Santiago had filed a temporary injunction against her ex-boyfriend, Baumet, on October 5. Santiago said Baumet went to her place of employment and she told him he was not allowed to be there.
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Baumet was scheduled to be in court yesterday at 1 pm (local time) to make the injunction permanent.
Heather Smith with the Seminole County Sheriff's Office said Baumet after shooting in the salon drove to a friend's house and shot and killed himself.
"Shortly after 11 this morning, we received a call from the residents inside this home. A man, who is the suspect in the Casselberry shooting, came to the residence and is friends with the couple who lives here," Smith said.
"He advised them he was having some problems and that they were problems he could not remedy and he proceeded to take his life with a firearm inside the home," he said.
The incident comes after several mass shooting incidents in the country.
On August 13, a man gunned down two people, including a constable, near a Texas university and was himself killed in the shooting incident,
A gunman identified as James Holmes opened fire in a movie theatre in Aurora, Coorado, at a midnight showing of The Dark Knight Rises on July 20, leaving 12 people dead.
On August 5 in Oak Creek, Wisconsin, a gunman with ties to white supremacists walked into a gurdwara and killed six Sikhs.