The 21-year-old "Drag Me Down" hitmaker tripped and lost his footing in front of the audience before falling flat on his back, reported E! Online.
Styles previously dragged his microphone and fell over backwards in San Diego during the opening night of the North American leg of One Direction's "On the Road Again" world tour.
The band, which now consists of Styles, Liam Payne, Louis Tomlinson and Niall Horan, also released the music video for their single "Drag Me Down," their first music video without former bandmate Zayn Malik.