Taking to Twitter, the minister said, "I strongly condemn the murder of Polio workers in Pakistan. Put your guns down today, or crutches is all that'll be left of your tomorrow."
A polio worker was gunned down on Saturday by two unidentified men in a northwestern province of Pakistan where the crippling disease remains endemic.
Sohail Ahmed, a technician of the Expanded Programme on Immunisation (EPI), was shot by motorbike riders armed with assault rifles while he was returning to his base from an outreach campaign in Swabi district.
Islamist militants consider anti-polio vaccines as a Western conspiracy to sterilise Muslims. More than 100 people have been killed in such attacks since December 2012.