Raj is the president of Maharashtra Navnirman Sena (MNS).
Justice Gautam Patel, on August 3, rejected Jaidev's application saying the video clip cannot be admitted as evidence as he is not its author and it was only uploaded on a social media site.
"It is inadmissible in evidence simply on production by the defendant (Jaidev), as he is not the author or creator of that video clip. The very fact that it was uploaded on YouTube does not mean its contents are true. Uploads and downloads do not establish the correctness of contents," Justice Gautam Patel said in his order.
Jaidev disputed the will alleging his father was of unsound mind and that Uddhav influenced him.
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The suit was filed after Bal Thackeray's death in November 2012. Recently, Jaidev was cross-examined by Uddhav's lawyer. The court midway directed that the proceedings be held in-camera.
Earlier, the court had asked the parties to settle the dispute, but it was not resolved.
D'Souza told the court that Thackeray had made multiple wills, in all of which the beneficiaries remained the same, but the assets willed to each beneficiary kept changing.
Jaidev had challenged Bal Thackeray's last will made on
December 13, 2011 which did not give him anything but bequeathed a substantial share of the property to his brother and incumbent Sena president Uddhav Thackeray.
Jaidev, whose relations with Bal Thackeray were strained, alleged that his father was of unsound mind and under Uddhav's influence when he made the will.
The high court had earlier asked the two parties to try and settle the dispute amicably, but they failed to reach a compromise.
A witness had told the court earlier that Bal Thackeray had made several wills since 1997. In all those wills, the beneficiaries remained the same but the assets given to each beneficiary kept changing.