A bench of Justice V P Vaish, before whom Tytler's plea challenging the CBI court order for reopening the probe against him is pending, refused to grant stay.
It also rejected Tytler's plea for fixing his appeal for hearing in March considering the elections and fixed the hearing for April 17.
On April 10, a trial court had ordered that the case be reopened against Tytler and also set aside the CBI closure report which gave the Delhi Congress leader a clean chit on the ground that there was "no evidence" against him.
Tytler is accused of instigating a mob that led to the murder of three men who had taken shelter at the Gurdwara on November 1, 1984.
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The mob attack was part of violence against Sikhs after the assassination of the then Prime Minister Indira Gandhi on October 31, 1984.
Three men Badal Singh, Thakur Singh and Gurcharan Singh were killed near Gurudwara Pul Bangash.
Tytler's role in the killing of the three men was re-investigated by CBI after a court had in December 2007 refused to accept the agency's closure report.
It added the agency had already re-investigated the case on the order of a trial court but there was insufficient evidence against Tytler.
Tytler was given a clean chit by CBI on April 2, 2009.