As part of the licensing agreement with Disney India's Consumer Products arm, Hero Cycles will sell 12 models, priced between Rs 3,300 to Rs 4,500.
These cycles, inspired by Disney and Marvel stories and characters, will be available across more than 100 premium distributors.
The range, featuring Disney franchises like Mickey & Friends, Disney Princesses, Disney.Pixar Cars and Marvel's Spider-Man, will be made available across 30 cities in the first three months.
The companies did not disclose the revenue sharing details.
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In the first phase, Hero will manufacture one lakh Disney and Marvel branded bicycles in the first year. It will be scaled up to five lakhs in the second year and a million cycles by the third year.
The kids bicycle segment is one of the fastest growing markets in India at 15 per cent annual growth. The overall market of kids bicycles, including organised and unorganised, is around 3 million units.