Listeners woke up yesterday morning to the Mandarin- language broadcast of the China National Radio Hong Kong Edition (CNR), instead of the World Service, which had been relayed live by Radio Television Hong Kong (RTHK) since 1978.
The BBC said it was "always disappointed when a service our listeners are used to changes" with listeners launching a petition to bring back the World Service.
RTHK has a number of different channels offering some programmes in English. The World Service was broadcast on Channel 6, which is now playing CNR.
Only some of its content is in Cantonese, which is the dominant language of Hong Kong, leading to criticism that this was another step towards the "mainlandisation" of Hong Kong.
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RTHK is still running a reduced version of the World Service on a different channel, but only late at night, from 11pm to 7am.
China stands accused of tightening its grip on Hong Kong, with critics also blaming the pro-Beijing local government for acting as a puppet.
The jailing of prominent young pro-democracy activists last month and the unveiling of a controversial rail link to the mainland that would see a portion of the city come under Chinese law have worsened fears the city's cherished freedoms are being eroded.
"The removal of the BBC World Service from the airwaves makes the city feel more parochial and inward-looking," the petition said.
Longtime resident Alex Hofford, who organised the petition, said he had nothing against the CNR broadcast but does not believe it should have come at the expense of the BBC.
"This is a sad day for Hong Kong, I'll really miss the Beeb as I drive around Hong Kong during the day," Hofford said.
RTHK's head of corporate communications Amen Ng told AFP today that it was a "difficult decision" due to "limited radio frequency".
"This is a cultural exchange between mainland China and Hong Kong," Ng added.
Hong Kong was handed back to China by colonial ruler Britain in 1997 under a "one country, two systems" agreement designed to protect its freedoms and way of life, but there are growing concerns those rights are now under threat.