The controversial infrared camera system has been dropped by the host broadcaster due to costs, Hot Spot's Australian inventor Warren Brennan told Fairfax Media.
The decision-review device, which uses heat sensors and infrared cameras to detect contact between the ball, bat and pads, will now not feature in the showpiece five-Test series, beginning at Brisbane's Gabba ground on November 21.
"It's their decision and that's what's been communicated to us. As far as I'm concerned, it is final," Brennan said.
The development leaves the ball-tracking component, Eagle Eye, audio evidence picked up by stump microphones and slow-motion replays as the remaining tools at the disposal of the third official for decision reviews in the Ashes.
More From This Section
Trust in the Hot Spot technology was severely tested during the first leg of the back-to-back Ashes series won 3-0 by England in July-August.
While match umpires were criticised for poor decisions, Hot Spot was at the centre of a succession of contentious verdicts involving fine edges.
A Cricket Australia spokesman said of the reports: "We don't think it's appropriate to comment on discussions between Nine and one of its partners.