The "bong" of Big Ben is produced when its huge 200kg hammer hits the side of the bell, setting off vibrations in the metal and causing the entire bell to pulsate, researchers said.
Officially known as the Great Bell, Big Ben is the largest of five bells that hang in the belfry of Elizabeth Tower at the Palace of Westminster.
It is thicker than other bells of a similar size, weighing more and as a result having a higher pitch than expected, said Martin Cockrill from the University of Leicester, who led the study.
They used two lasers to scan Big Ben as it chimed at 9, 10, 11 and 12 O'clock, 'BBC News' reported.
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Scientists employed a technique called "laser Doppler vibrometry," which involved creating a 3D computer model of Big Ben.
Two lasers were then used to map the vibrations in the metal of the bell as it chimed.
The researchers have produced animations showing the different vibration patterns of the bell.
Big Ben was cast at the Whitechapel Bell Foundry in 1858. It weighs 13.7 tonnes and is one of the largest bells in the UK.