Photojournalist Alfred Eisenstaedt took the photograph as news broke that Japan had surrendered on August 14, 1945, bringing to a close the most brutal conflict the world has ever known.
The photo, known as "V-J Day in Times Square" for Victory over Japan, shows an American sailor kissing a woman in white, full on the mouth as her right foot rises off the ground.
Some of Friday's couples turned out in replica outfits, the men dressed in sailor suits and the women in white.
World War II veterans Ray and Ellie Williams, who got married on August 15, 1945, were guests of honor and flew in from the southern US state of Georgia for the event.
More From This Section
Ray Williams said he remembered the joy and jubilation that greeted the end of the war.
"We experienced that joy, it was a Friday evening. Jubilation broke out all over the country," he said. "We were double excited, celebrating the end of the war and also our marriage."
A sculpture of the kiss was also installed in Times Square for the occasion.
Eisenstaedt's photograph was published in Life magazine. He was in so much haste to cover the breaking news that he did not stop to ask the name of the couple.
Their faces are not clearly visible in the photograph and their identity remains a mystery. Eleven men and three women have since come forward to claim they are in the photograph.
Japan surrendered on August 14, 1945 after America dropped two atomic bombs, on Hiroshima et Nagasaki, which killed more than 100,000 people instantly.