By organising a mass hunger strike by hundreds of Palestinian prisoners, Barghouti has solidified his standing as a likely successor to President Mahmoud Abbas.
He also has proven to Israel that, despite serving multiple life sentences, he cannot be ignored as the country marks 50 years of control over the Palestinian territories.
Barghouti is the most prominent of the thousands of Palestinians held in Israeli prisons, and even after 15 years behind bars, he continues to cast a long shadow over the region.
He has called the new hunger strike the "long walk to freedom," the title of Mandela's autobiography about life in prison during the apartheid era.
More From This Section
"Marwan is the best candidate to inspire and lead the new generation from behind bars, just as Mandela did," said Qadura Fares, a Barghouti supporter and leader of the Palestinian prisoners' association.
Palestinian officials say Israel is holding some 6,500 "security prisoners" - people jailed for charges ranging from stone-throwing and membership in groups outlawed by Israel, to attacks that wounded or killed Israelis. Several hundred are being detained without charges.
Barghouti did not offer a defense, refusing to recognize the court's authority.
As many as 1,500 prisoners are believed to have joined the strike. Their immediate demands included better conditions, more contact with relatives, and an end to Israel's practice of detentions without trial.
But the strike's significance runs far deeper, with long-ranging implications in the brewing struggle to succeed the 82-year-old Abbas and efforts to end a bitter 10-year rift between Abbas' Fatah movement and the rival Hamas militant group.
Over the years, polls have indicated that Barghouti is the most popular choice among Palestinians to succeed Abbas, who has refused to groom a political heir.
He also is seen as the only figure in Abbas' Fatah movement who also enjoys support from Hamas, which seized control of the Gaza Strip from Abbas' forces a decade ago. The ongoing rift is a major impediment to Abbas' goal of establishing a Palestinian state in both territories.