The 23-year-old, who went on a hunger strike to protest what she described as "slave labour conditions" and death threats, was moved yesterday from her Mordovia prison colony to a nearby hospital.
Her husband, activist Pyotr Verzilov, said the authorities of the hospital, which is part of the prison system, denied his request to meet with Tolokonnikova.
"Colonel Oleg Klishkov, head of medical facility number 21, officially told me that he was refusing me a meeting with Nadia," Verzilov told AFP, referring to his wife by her nickname.
"They explained it by the fact that her state of health is so bad that she cannot speak with her defence."
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Yesterday, Verzilov issued an open letter addressed to the head of the Federal Service for the Execution of Punishment, which oversees prisons, protesting that Tolokonnikova was being held incommunicado.
Tolokonnikova went on a hunger strike on September 23, releasing an open letter in which she described harrowing conditions at her prison and alleged that she had received death threats over her complaints.
On the fifth day of her hunger strike Friday, Tolokonnikova was moved to the medical unit of her penal colony after her health worsened.
Tolokonnikova is serving a two-year sentence for performing a protest song against President Vladimir Putin in Moscow's main cathedral last year.