Parton, 68, said that despite her successful career in country music, she wants a serious film role, reported Digital Spy.
Parton has previously starred in movies including '9 to 5', 'Rhinestone' and 'Miss Congeniality 2', but wants to lose her trademark denim, big hair and make-up and build a credible reputation as an actress.
"I would someday love to find a really great role in a movie. One where I didn't wear the nails, the high heels, the make-up. I would love to play a part that was worth tearing all that stuff away for," she said.
However, the 'Jolene' singer admitted that she would not find it easy to change her image for an acting part, as it has played such a big part in her life.
"I would not be comfortable. But if the role was good enough, I'd be willing to do that. To play something so different that it would begin to make sense. I'd be able to get comfortable with it.