Cho's avatar as General Cho Young Ja at the ceremony drew some angry comments on Twitter with many viewers feeling that she was playing up to the Asian stereotype.
But the comedian said the joke should not be taken so seriously.
"I'm not playing the race card. I'm playing the rice card," Cho tweeted in response to the criticism.
"I'm of mixed North/South Korean descent - you imprison, starve and brainwash my people you get made fun of by me #hatersgonhate #FreeSpeech," Cho explained.
But Cho's fans thought otherwise as they posted their reaction.
"I've always loved margaret cho but she needs to stop shuckin' for white folk," one user wrote.