The announcement was made after a meeting of Prime Minister Narendra Modi and his Australian counterpart Malcolm Turnbull here on the sidelines of the G20 Summit.
"Another milestone achievement as two PMs announce completion of procedures for India Australia Civil Nuclear Agreement," External Affairs Ministry spokesperson Vikas Swarup tweeted after the meeting.
"With the completion of procedures, including administrative arrangements, the India Australia Civil Nuclear Agreement will enter into force," he said.
Australia had signed a civil nuclear agreement with India in September last year to supply it uranium for meeting Indian energy needs.
Modi thanked Turnbull and described the agreement as a "milestone and source of trust and confidence".