The move is aimed at discouraging imports of natural rubber.
"Import of natural rubber of all varieties/forms ... Is allowed only through sea ports of Chennai and Nhava Sheva (Jawaharlal Nehru Port)," the Directorate General of Foreign Trade (DGFT) said in a notification.
Domestic producers have time and again raised concerns over increasing rubber imports into the country and dip in prices due to that.
United Planters Association of Southern India (UPASI) has said that cheap imports are harming India's rubber industry and the government should introduce safeguards to protect domestic players.
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India's natural rubber imports have declined by 9 per cent to 2.88 lakh tonnes in the first eight months of the current financial year.
In a separate notification, the DGFT said that export of roasted Gram (whole/split) in consumer packs of one Kg has been permitted.
The prohibition on export of pulses would not apply to export of roasted gram in consumer packs of one Kg, it added.