"The number two official in Bahrain's embassy in Iran is persona non grata and Mr Bassam al-Dossari must leave Iran's territory within 72 hours," the official IRNA news agency quoted a foreign ministry statement as saying late yesterday.
Bahrain on Thursday recalled its ambassador from Iran and ordered Tehran's envoy to leave within 72 hours, citing Iranian "interference" in its affairs.
The Gulf kingdom of Bahrain, with a majority Shiite Muslim population ruled by a Sunni dynasty, has suffered unrest since a pro-democracy uprising in 2011.
The kingdom is "evading its internal problems by passing the buck, instead of solving them" Iran's Deputy Foreign Minister Hossein Amir Abdollahian told state television on yesterday.
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"Tehran has not and will not interfere in Bahrain's internal affairs and reacts accordingly to Bahrain's actions."
Yesterday, Bahrain took its protest to the United Nations by submitting a complaint against Iran.
"Iran has chosen the path of escalation in an attempt to exert control over its neighbours by continuing to interfere in internal affairs" of neighbouring countries, BNA news agency quoted Foreign Minister Khaled bin Ahmed al-Khalifa as telling UN chief Ban Ki-moon.
The coalition accuses Iran of arming rebels in Bahrain and Yemen.