Whoever is elected president on June 14 to succeed Mahmoud Ahmadinejad, the Islamic republic is unlikely to alter the course of its controversial programme of uranium enrichment.
Supreme leader Ayatollah Ali Khamenei takes the key decisions in Iran, including on the nuclear issue.
Western powers believe Iran's nuclear activities may have a covert military purpose, but Tehran denies this, saying they are entirely peaceful.
"Definitely the result of the presidential election will not have any influence on the nuclear issue," the country's atomic chief Fereydoun Abbasi Davani has said.
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"Regardless of who is elected president in June, uranium enrichment activities will be pursued without fear against the enemy," Jalili said.
"The president must demonstrate this in a practical manner to the supreme leader," Jalili, who has been negotiating with world powers on the issue since October 2007, said on his campaign website.
Neither the United States nor Iran's regional arch-rival Israel has ruled out taking military action against Iranian atomic facilities over fears that they mask a secret nuclear drive, despite the denials.
Since 2003, Tehran has been engaged in talks with not only UN watchdog the International Atomic Energy Agency (IAEA) but also with world powers to try to resolve the issue.
Tehran maintains that as a member of the nuclear Non-Proliferation Treaty (NPT) it is within its rights to run a nuclear programme to generate electricity and for medical purposes.
Khamenei and other senior officials have repeatedly said that making, owning or using atomic weapons is "haram" -- forbidden under Islam.