The Olympic Council of Asia (OCA) banned football equipment manager Amereh Ahmad from Games sites and asked the Iranian team to send him home for verbally harassing the volunteer on Monday.
Also on Wednesday, police were investigating a Palestinian footballer who was accused of groping a female worker in an athletes' village laundry room the day before. The player was banned from leaving the country.
The incidents prompted the organising committee to put up signs in Games accommodation warning against harassment, while the OCA said it had "zero tolerance" for such cases.
"The OCA will not tolerate any misbehaviour of this nature and we will take a tough stance. We cannot accept this, so we have removed him from the Games."
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In a third case related to the Asian Games which formally open on Friday, an Indian gymnast and his coach were under investigation for allegedly harassing a female athlete at a pre-Asiad camp in New Delhi.
The Incheon Asian Games Organising Committee (IAGOC) put up strongly worded posters warning that harassment cases would be prosecuted under South Korean law.
"Sexual violence includes any behaviour that causes sexual humiliation through physical contact.
"The assailant will definitely be punished and may bring down the virtue of his/her nation.