The propaganda video by the IS-affiliated Amaq network shows an emaciated Cantlie speaking in English to the camera in the style of a news report similar to previous videos in which he appeared.
The footage was not dated but the destroyed bridges seen behind Cantlie in the video suggest it was shot in recent weeks.
The US-led coalition assisting Iraq in its fight against IS has destroyed several bridges in central Mosul over the past few weeks.
Cantlie was kidnapped along with fellow journalist James Foley while covering the war in Syria in November 2012.
Foley then became the first of several hostages to be slain by IS.
Media rights watchdog Reporters Without Borders has condemned IS for its "cowardly" use of a hostage in a forced role to promote its propaganda.
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