A Palestinian security source confirmed that "around 30 people" had been detained in and around the northern city of Nablus, most of whom were Hamas members.
Among those arrested were university students and a journalist. Troops also confiscated computers and mobile phones, the source said.
The Israeli army confirmed arresting 29 people, among them senior figures within the movement and former prisoners detained for involvement in Hamas activity, in a joint operation which involved soldiers, police, border police and the Shin Bet internal security service.
The sweep raised to 49 the number of Hamas activists arrested by Israel since April 1, the army said.
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Since the kidnapping and subsequent murders of three Israeli teenagers in the southern West Bank last June, there has been a rise in the number of Hamas activists arrested by the army, with hundreds now behind bars.
In March, between 40 and 60 Hamas activists were arrested by Palestinian security forces.