Wednesday's surprise deal, which saw Palestinian leaders from the West Bank and the Hamas-run Gaza Strip agree to work together after years of bitter rivalry, was denounced the next day by the Israeli security cabinet.
That cabinet said it would "not negotiate" with any Palestinian government backed by the Islamist movement.
In an address to PLO leaders on Saturday, Palestinian president Mahmud Abbas said the new government, which will be made up of political independents, would recognise Israel, reject violence and abide by existing agreements.
"Hamas denies the Holocaust while attempting to carry out a second Holocaust by destroying the state of Israel," he said. "It is with this Hamas that Abu Mazen (Abbas) chose to make an alliance."
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"Abu Mazen must decide between an alliance with Hamas -- a terrorist organisation which denies the Holocaust -- and real peace with Israel."
Justice Minister Tzipi Livni, Israel's chief negotiator with the Palestinians, said however that it was crucial to wait and see what sort of government emerged.
Livni ruled out any talks with Hamas and said the international community must demand that the emerging Palestinian leadership "adopt the requirements" of the Middle East peacemaking Quartet.
"I shall not conduct negotiations -- direct or indirect -- with Hamas," she said, suggesting that the peace process was not yet over.
The Quartet demands that Hamas recognise Israel, renounce violence and abide by existing agreements between Israel and the PLO.
Finance Minister Yair Lapid also said it was too early to call time on the negotiations.
"If Hamas accepts the Quartet conditions... It will not, in effect, be Hamas any longer and then there'll be a basis for discussion," he told public radio.
"We don't see it at the moment, but we need to watch and wait and study what's going on."
Economy Minister Naftali Bennett, who has stubbornly opposed the US-led peace talks, ruled out any dialogue with groups that have taken part in anti-Israeli attacks.