"I believe that it should be in the first priority of each of us to deal with this threat by all means, in all fields," Moshe Yaalon said at joint press conference with visiting US Defence Secretary Chuck Hagel.
The Pentagon chief's visit came as the United States and other major powers pressed talks with Iran on a long-term agreement to allay international concerns about its nuclear ambitions.
Israel has strongly opposed the negotiations with its arch-enemy, and has said repeatedly that it is prepared to go it alone if necessary with preemptive military action against Iran's nuclear facilities.
"We might have differences, even disputes regarding how to get it but we have the open channels, the secretary of defence and myself.
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"The bottom line is that Israel should be ready to defend itself, by itself."
Hagel said the negotiations between the powers and Iran were not open-ended -- the parties are working to a July 20 target for an agreement.
He said Washington was continuing to cooperate closely with its Israeli ally on the Iran issue, even while the negotiations continued.
Hagel was on the last leg of Middle East, which also took him to Saudi Arabia, which has its own concerns about the nuclear talks with its regional rival.