Pratt, 35, commented on the rumour during the Hasty Pudding Man of the Year award, reported Aceshowbiz.
He said it would be an 'awesome opportunity' to portray the role of archaeologist Dr Henry 'Indiana' Jones, which was previously made famous by Harrison Ford.
It was reported last month that Pratt was wanted to play the character in 'Indiana Jones V'.
Pratt was at that time asked about the story at an airport, shortly after it broke out. "I don't know, I just got back from the woods killing a bunch of stuff," he said while gesturing to his luggage.