The unnamed film will feature Daniel Craig as the British secret agent for a fourth time after his exploits in 'Casino Royale', 'Quantum of Solace' and 'Skyfall', reported Daily Express.
Bond will reportedly drive the Fiat during a high-speed car chase through the street of Rome, with scenes due to be shot in February and March next year.
But the Italian vehicle will apparently end up being crushed near the Vatican.
His Aston Martin DB5 came to an unfortunate end in 'Skyfall', where it was blown up in the climax to the film.
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It featured for the first time in the third 007 film, 'Goldfinger' (1964) when Sean Connery was Bond - and a DB5 was also in Craig's first outing in 'Casino Royale'.
Rome will host three key action scenes for the new movie, including night-time chases, with one car ending up in the Tibur River.
Bond will parachute from a helicopter onto Ponte Sisto, a 15th-century pedestrian bridge. There will also be scenes shot outside of Rome - in the Royal Palace of Caserta, known as the Versailles of Italy, in Campania.
The new movie, directed by Sam Mendes who was also at the helm for 'Skyfall', is expected to hit cinemas late next year.