Timberlake will be back to perform during the halftime of Super Bowl LII on February 4.
His performance comes 14 years after the controversial halftime show with Jackson, in which he ripped off a portion of her costume, that partly revealed her breast.
Jackson released a statement ahead of the big game and denied the rumours that she is going to perform at the event.
"To put to rest any speculation or rumours as to whether I will be performing at the Super Bowl tomorrow: I will not," Jackson said in the statement, according to People magazine.