Party chief whip Shrawan Kumar wrote a letter to Speaker Udai Narayan Choudhary for termination of the membership of Ajit Kumar.
Ajit Kumar is member of the Legislative Assembly from Kanti in Muzaffarpur district and has served as a minister in the Nitish Kumar cabinet from 2005 to 2010.
He is the fifth rebel JD(U) MLA against whom the party has cracked whip and recommended cancellation of membership.
The other four - Gyanendra Singh Gyanu, Ravindra Rai, Neeraj Singh Babloo and Rahul Kumar - have to reply to the show cause in this regard to the Speaker on July 5.
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"This is violation of the Constitution's 10th schedule relating to anti-defection law," he said justifying the action against Ajit Kumar.
Shrawan Kumar had send a report against 18 JD(U) dissidents to the party leadership for disciplinary action for working in favour of Independents against the party's official candidates in the Rajya Sabha bypoll.