Emmy, Golden Globe and SAG Awards winning actor has been accused of acting inappropriately around his former assistant Van Barnes, who made her allegations in a private Facebook post, reported Deadline.
Tambor, 73, has denied the allegation in a statement to the Deadline, calling Barnes a "disgruntled assistant".
"I am aware that a former disgruntled assistant of mine has made a private post implying that I had acted in an improper manner toward her," said Tambor.
Meanwhile, Amazon Studios which produces "Transparent", has initiated an investigation into the claims of Barnes.
The studio is already hit by a harassment case involving its former vice president, Roy Price, who was suspended by following a harassment claim from one of the producers.