Anthony will act as executive producer via his company Magnus Media and Sony Music Latin for Lopez's second Spanish language album which is slated for a November release, reported People magazine.
"I am so excited and really looking forward to this new musical journey and to celebrate my Latin roots with Marc Anthony and the Sony/Magnus family," the 47-year-old songstress said in a statement.
The duo, who share 8-year-old twins Emma and Max, have remained friendly in the five years since they split and even shared the stage in New York City earlier this summer.
"Her artistry, professionalism and passion for music are just some of the many attributes that have earned her a unique place in the entertainment world that very few people have been able to obtain. I am looking forward to our creative collaboration and to be part of continuing her musical legacy, Anthony, 48, said.
JLo released her first Spanish album "Como Ama una Mujer", which was also co-produced by Anthony back in 2007.