Led by General Secretary Paramjit Singh, over 250 staffers, including drivers and conductors, assembled at the organisation's headquarters here where they sat on a dharna against the government's alleged failure to grant them benefits under the 6th Pay Commission.
Both intra and inter-state public transport services were affected by the strike.
Singh told reporters that their protest was also over the government's non-payment of dearness allowance for years.
"Unless the government fulfils our demand within a week, we will resort to an indefinite strike. The government will be responsible for losses and problems caused to people on account of the strike," Singh warned.