The 37-year-old actress attended 2013 Women in the World summit at Lincoln Center in New York City on April 4 along with actress Meryl Streep, reported Ace Showbiz.
"Here's what they accomplished," Jolie, who is the special envoy for the UN refugee agency, said of Yousafzai's attackers in the emotional tribute to introduce the little girl to the crowd.
"They shot her point blank range in the head - and made her stronger. The brutal attempt to silence her voice made it stronger," she added.
"So there's a lot we can learn from this little girl," Jolie said.
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The inspiring teen then appeared via a video from Britain, where she underwent surgery and skull reconstructions and now attends school.
Jolie announced the Malala Fund and said a school had been built in her home country for 40 girls, thanks to the charity.
"Let us turn the education of 40 girls into 40 million girls," she said.
In another tribute segment, Streep took the stage to honour another activist, Inez McCormack of Northern Ireland, who died in January of cancer.