"Jordan conducted an air strike overnight," said one of the officials, who spoke on condition of anonymity.
The raid was in addition to a US bombing run announced earlier by the American military's Central Command, the official said.
Pentagon officials offered no further details on the Jordanian air strike.
Jordan confirmed yesterday that it was taking part in the air campaign and four other Arab states have said they are participating: Bahrain, Qatar, Saudi Arabia and the United Arab Emirates.
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US aircraft have been bombing IS extremists in Iraq since August 8. The bombing runs continued into today, with four raids bringing the total number of air strikes in Iraq to 198, according to US Central Command, which oversees American forces in the Middle East.
France recently joined the air campaign in Iraq.
In the latest air raids in Iraq, strikes west of the capital Baghdad hit two IS armed vehicles and an arms dump. Two more destroyed IS fighting positions threatening the Kurdish regional capital Arbil, Central Command said.