Louis-Dreyfus, 55, won the best the best actress in a comedy series award for her performance on "Veep".
"I'd like to dedicate this to my father, William Louis-Dreyfus, who passed away on Friday," she said at the end of her otherwise light-hearted speech.
"I'm so glad he liked 'Veep' because his opinion was the one that really mattered."
The moving tribute by the star brought audience members to tears as well. The cameras flashed to Jerry Seinfeld, her longtime co-star on "Seinfeld", who was also visibly moved.