The doctors were on strike since Wednesday after a doctor at the hospital was heckled by relatives of a patient who died at the hospital.
Following the stir, Chief Minister Mamata Banerjee asked the health department to sort out the matter and restore normal services at the hospital, Health Secretary Sanjay Mitra told PTI.
The ceasework was lifted after three persons were arrested, he said.
The health department had earlier cancelled leave of all senior doctors at the hospital who were deployed to man emergency services.
Many patients were shifted to other hospitals following the ceasework at north Kolkata's largest state-run hospital.
Minister of state for Health Chandrima Bhattacharya had yesterday asked the junior doctors to return to work in the interest of the patients.