Thomas Carballo, the host of the show, was in the middle of a phone chat with the pop star when Bieber took offence at his line of questioning - and hung up, reported Daily Express.
The 10-minute interview got off to a bad start when Carballo told his guest he thought he was Timberlake the first time he heard him.
To which Bieber replied, "That's funny because our voices sound nothing alike... Saying I sound like somebody else is definitely not a compliment."
The singer then started talking about his new song 'Maria', which was inspired by the woman who falsely claimed Bieber was the father of her baby last year.
But the 19-year-old lost his cool when Carballo got personal and asked if he was worried about his One Direction friend Harry Styles falling for his mother.
Bieber then mumbled something about Carballo's mother and, as the radio presenter explained his mother was dead, the pop star hung up the phone.